{"id":1596,"date":"2018-04-04T12:46:34","date_gmt":"2018-04-04T04:46:34","guid":{"rendered":"https:\/\/apcf-infosite-dev.hkust.edu.hk\/?page_id=1596"},"modified":"2024-10-10T17:09:49","modified_gmt":"2024-10-10T09:09:49","slug":"pronuclei-dna-or-crispr-cas9-components-microinjection-2","status":"publish","type":"page","link":"https:\/\/apcf-infosite.hkust.edu.hk\/?page_id=1596","title":{"rendered":"Pronuclear (DNA or CRISPR\/Cas9 components) Microinjection"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1595 alignleft\" src=\"https:\/\/apcf-infosite-dev.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Embryos.jpg\" alt=\"\" width=\"580\" height=\"366\" srcset=\"https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Embryos.jpg 2039w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Embryos-300x190.jpg 300w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Embryos-768x486.jpg 768w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Embryos-1024x647.jpg 1024w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Embryos-1200x759.jpg 1200w\" sizes=\"(max-width: 580px) 85vw, 580px\" \/><\/p>\n<p style=\"text-align: left;\"><em>Transgenic mice generated by pronuclear DNA microinjection. EGFPcre<\/em> expression driven by a transcriptional enhancer element to reveal gene expression pattern and to establish tissue specific knockout of genes by <em>cre\/loxP<\/em> systems (<em>Siva Tsang<\/em>)<\/p>\n<hr \/>\n<h6>Services include:<\/h6>\n<ul>\n<li>Preparation of pseudopregnant surrogates<\/li>\n<li>Superovulation of donor females<\/li>\n<li>Production of zygotes<\/li>\n<li>Microinject at least 200 zygotes<\/li>\n<li>Surgical transfer of all injected zygotes into the surrogates<\/li>\n<\/ul>\n<p>Fee: HKD 10,000<\/p>\n<h6>Contacts:<\/h6>\n<p>Dr. Siva WH Tsang (email: tsangwh@ust.hk; tel: 3469 2418)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Transgenic mice generated by pronuclear DNA microinjection. EGFPcre expression driven by a transcriptional enhancer element to reveal gene expression pattern and to establish tissue specific knockout of genes by cre\/loxP systems (Siva Tsang) Services include: Preparation of pseudopregnant surrogates Superovulation of donor females Production of zygotes Microinject at least 200 zygotes Surgical transfer of all &hellip; <a href=\"https:\/\/apcf-infosite.hkust.edu.hk\/?page_id=1596\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Pronuclear (DNA or CRISPR\/Cas9 components) Microinjection&#8221;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages\/1596"}],"collection":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1596"}],"version-history":[{"count":8,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages\/1596\/revisions"}],"predecessor-version":[{"id":3754,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages\/1596\/revisions\/3754"}],"wp:attachment":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1596"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}