{"id":1603,"date":"2018-04-04T12:55:44","date_gmt":"2018-04-04T04:55:44","guid":{"rendered":"https:\/\/apcf-infosite-dev.hkust.edu.hk\/?page_id=1603"},"modified":"2024-10-10T17:11:15","modified_gmt":"2024-10-10T09:11:15","slug":"embryo-cryopreservation","status":"publish","type":"page","link":"https:\/\/apcf-infosite.hkust.edu.hk\/?page_id=1603","title":{"rendered":"Embryo Cryopreservation"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1551 alignleft\" src=\"https:\/\/apcf-infosite-dev.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Cryopreservation.png\" alt=\"\" width=\"630\" height=\"475\" srcset=\"https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Cryopreservation.png 1110w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Cryopreservation-300x226.png 300w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Cryopreservation-768x580.png 768w, https:\/\/apcf-infosite.hkust.edu.hk\/wp-content\/uploads\/2018\/04\/Cryopreservation-1024x773.png 1024w\" sizes=\"(max-width: 630px) 85vw, 630px\" \/><\/p>\n<p style=\"text-align: left;\">Physical responses of mouse embryos under treatments with cryoprotectants (required before rapid-freezing) and rehydration (required in the recovery processes) (Siva Tsang).<\/p>\n<hr \/>\n<h6 style=\"text-align: left;\">One session of services includes:<\/h6>\n<ul>\n<li>Superovulation of donor females (if applicable)<\/li>\n<li>Harvesting embryos from up to 8 donor females<\/li>\n<li>Cryopreserving all harvested embryos by vitrification<\/li>\n<\/ul>\n<p>Fee: HKD 2,000 \/ session (+HKD 500 per 5 years\u00a0 storage per strain)<\/p>\n<p>Notes: Transgenic strains may have variable mating performance and productivity of embryos. No. of embryos to be cryopreserved per service can vary.<\/p>\n<h6>FAQ: How many embryos I need?<\/h6>\n<p>It depends. One may consider the following factors.<\/p>\n<ul>\n<li><strong><em>The availability of backup supply of the strain.\u00a0<\/em><\/strong> If a strain is not available elsewhere from a reliable reposition, and no other similar strains available, more embryos may be stocked to manage the risk of losing the strain from the planet.<\/li>\n<li><strong><em>Plan to redistribute the strain.<\/em> <\/strong>If one is prepared to redistribute the strains to collaborators or other requesters,\u00a0more embryos may be stocked in advance.<\/li>\n<li><strong><em>Predicted genotype distribution among the thawed embryos<\/em><\/strong>. In a Hetero x WT cross to produce embryos for cryopreservation, one may expect half of the embryos are hetero. One will therefore double the number of embryos when comparing with a Homo x WT strategy.<\/li>\n<\/ul>\n<p>One may also consider the following practical factors.<\/p>\n<ul>\n<li>We put max 25 embryos in one container, thus thawing 25 embryos at a time.<\/li>\n<li>More than 95% of embryos can be recovered in vitro and half of the embryos can develop to term after surgical transfer (similar to non-cryopreserved, fresh embryos).<\/li>\n<li>Surrogates occupationally fail to maintain pregnancy,\u00a0naturally, after surgical transfer of embryos.<\/li>\n<li>One may want the revived animals be males to speed up the expansion of the colony.<\/li>\n<\/ul>\n<p>Please contact us for further advise.<\/p>\n<h6>Contacts:<\/h6>\n<p>Dr. Siva WH Tsang (email: tsangwh@ust.hk; tel: 3469 2418)<\/p>\n<h6>Reference:<\/h6>\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19594455\" target=\"_blank\" rel=\"noopener noreferrer\">Tsang WH and Chow KL (2009).\u00a0Mouse embryo cryopreservation utilizing a novel high-capacity vitrification spatula.\u00a0Biotechniques. 2009 Jun;46(7):550-2. doi: 10.2144\/000113125.<\/a><\/p>\n<p><a href=\"https:\/\/mts.intechopen.com\/books\/current-frontiers-in-cryobiology\/cryopreservation-of-embryos-of-model-animals-and-human\" target=\"_blank\" rel=\"noopener noreferrer\">Tsang WH and Chow KL (2012). Cryopreservation of Embryos from Model Animals and Human, Current Frontiers in Cryobiology, InTech, DOI: 10.5772\/32803.\u00a0<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Physical responses of mouse embryos under treatments with cryoprotectants (required before rapid-freezing) and rehydration (required in the recovery processes) (Siva Tsang). One session of services includes: Superovulation of donor females (if applicable) Harvesting embryos from up to 8 donor females Cryopreserving all harvested embryos by vitrification Fee: HKD 2,000 \/ session (+HKD 500 per 5 &hellip; <a href=\"https:\/\/apcf-infosite.hkust.edu.hk\/?page_id=1603\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Embryo Cryopreservation&#8221;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages\/1603"}],"collection":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1603"}],"version-history":[{"count":19,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages\/1603\/revisions"}],"predecessor-version":[{"id":3756,"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=\/wp\/v2\/pages\/1603\/revisions\/3756"}],"wp:attachment":[{"href":"https:\/\/apcf-infosite.hkust.edu.hk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1603"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}